Isolation and Identification of Ethanol Extract Andrographis Paniculata Nees and Moringa Oleifera Lam

Sambiloto and Kelor had been used frequently to decrease disease simptoms and pain. It will be
correlated with the composition of some secondary metabolite compound and other chemicals in Sambiloto and
Kelor such as alkaloid, flavonoid and terpenoid. This study was to isolate and identified the secondary
metabolite of ethanol extract Andrographis paniculata Ness and Moringa oleifera Lam. The objective of this
study was to get substrate as alternative of nature material which easy to obtain, rationally and economics.
Sambiloto and Kelor extract prepared by maceration used ethanol 70%. Sambiloto and Kelor ethanol extract
(EES and EDK) then separated with thin layer chromatography (TLC) and identified with UV lamp. Then
activity test using GCMS. The most peak of Sambiloto in testing with GCMS was Phytol (C20H40O) which was
diterpen alcohol compound acyclic hydrogenated while in Kelor it was 9, 12, 15-Octadecatrienoic acid
(C18H30O2) which was linolenic acid. Targeted output of this study was a manuscript published at accredited
international journal. Technology Readiness Level in this study was in second level and after this study become
third level by doing some activities such as extraction, isolation, identification and activity test.